Photobioreactor Benefits over Traditional Open Pond Systems for the Creation of Bio Algae
The two primary methods currently available for growing and harvesting algae are open pond systems and closed system photobioreactors (PBR). PBR’s create an enclosed growing environment for algae cultivation where light, air, and nutrients are supplied at regulated levels to ensure optimized growth. The following bullet points illustrate the problems versus benefits between the two systems.
Problems with open algae systems (pond)
- Light only effectively penetrates 2’ – 3” in ponds resulting in large amount of algae not receiving enough light which lowers yields
- Temperature fluctuations can effect algae growths and yields
- Open to contaminates or more hearty local varieties of algae which could take over the pond requiring draining and/or treatment
- Excessive evaporation
Benefits of open algae systems (pond)
- Less expensive to create and maintain
Problems with closed loop algae systems (PBR)
- Capital intensive – more expensive to set up
- Facilities require greater amounts of maintenance
Benefits of closed loop algae systems (PBR)
- Controlled environment – species integrity can be maintained
- Productivity increases – able to monitor complete system more efficiently
- Less evaporation
- Interior lighting can be adjusted for proper exposure levels
The production cycle for growing algae and harvesting oil and biomass in a closed PBR system is as follows:
Algae strains are usually started in small containers in a laboratory and then the culture is either transported directly into a PBR or to shallow specialized raceway ponds that have paddle wheels to maintain water flow. If the raceway pond method is utilized the algae are then allowed to multiply in these artificial ponds and once a satisfactory density is reached it can then be transferred to the bioreactor. The algae/water mixture is then poured into the bioreactor system’s tank where it mixes with water, CO2, and nutrients already present in the system. CO2 and nutrients can also be introduced later in the system. The algae are then pumped into racks of translucent plastic containers. These containers may consist of long polyethylene bags, polyethylene sleeves, plastic tubes, or glass tubes. It is here the algae are exposed to light for photosynthesis. Pumps may continue to force the algae through the system or gravity may be used to allow the algae to flow down through the containers. Types of bioreactors include air lift, tubular, and flat plate.
There are two methods of operation, batch and continuous flow. In batch operations once the algae is ready for harvest, in some cases as quick as 48 hours, the entire PBR system is drained and algae is removed from the system and the PBR is restocked. In a continuous flow system only the excess mature algae are removed as the system becomes overloaded. Continuous flow systems can potentially run for very long periods. They may require new cultures to be introduced occasionally to re-kick start the system. Great care in monitoring must be taken to avoid a collapse of the entire algae colony within the system. If a collapse occurs it will require draining the system and starting over with a new culture. The advantage of the continuous flow is that air, CO2, nutrients, light levels, water mediums, and water temperature can be adjusted to create customized growing conditions. Cyanobacteria (blue-green algae) which excrete lipids (oils) as waste can also be harvested in this manner.
Algae can grow in a number of different water mediums including saltwater, brackish water, and waste water. It can also grow in a wide range of water temperatures. CO2 requirements can vary as well but when optimized can increase oil yields; the general rule of thumb is 2.2 lbs. of CO2 inserted into the system for every 1 lb. of algae for its lifecycle. The preferred method to increase CO2 solubility and oil yields is to use fresh water in moderate temperatures. Exposure to high (hot) water temperatures creates a metabolic burden in the algae that can slow growth rates. Lighting conditions are also critical to growth rates. Algae can grow successfully in different lighting levels. Bright light however, tends to degrade algal pigmentation and which can also lead to slower growth rates. 5% – 20% of full sunlight exposure subdues and preserves pigmentation creating a metabolic benefit that can lead to faster growth. This can easily be accomplished in a PBR system by adjusting internal lighting levels or by using plastic that are not 100% transparent or tinting in outdoor sections. Algae must be also be allowed a recovery period in darkness between 2 – 6 hours depending on species to allow for regeneration. Nutrient content and quantity can also be experimented with and adjusted depending on desired oil yield versus nutritional content in biomass residue.
When the growth cycle is completed and the algae colony has reached maturity it is ready for harvest (2 – 5 days dependant on species). The algae and water medium can be either completely drained from the system (batch mode) or harvested constantly in a continuous operation cycle. Operating in continuous cycle requires greater system monitoring and more precise administration of water, CO2, and nutrient levels but provides potentially greater yields. The algae can be harvested from the system by a number of different procedures or combination of procedures. The process usually involves some type of micro screening that allows water to pass through but retains the algae. This can be combined with centrifugation which involves high speed spinning and use of centrifugal force. Other methods include flocculation which uses chemicals or catalysts to promote formation of clusters which can then be easily gathered, or by froth flotation which involves grinding and crushing the algae repeated into froth and then skimming the surface for removal.
Once the algae has been dewatered and separated from the system it is allowed a period to dry. The lipids or oil must then be extracted from the dried algae. Again, there are a number of different methods available and can be used in combinations to increase efficiency. One of the simplest methods is using oil presses to crush the algae. There are a variety of methods used for crushing and pressing including screws, expellers, pistons, and other traditional presses that have been used successfully for extracting vegetable oils. A second method involves using chemical solvents such as hexane, benzene, and ether. These chemicals when introduced to the algae cause the cell walls to rupture releasing the oils. Another method involves using enzymes in a water medium to deteriorate the cell walls eventually requiring the oil to be removed from the water medium as it floats to the surface. With this process the alga doesn’t have to be removed from the PBR system via dewatering but can simply be transferred into another section for enzymatic extraction. Ultrasonic waves can be used in conjunction with enzymatic extraction to expedite the processes.
What are left are raw oil and a biomass residue. The oil can be refined to produce bio diesel, jet fuel, and pharmaceutical components. The biomass residue can be broken down into protein, carbohydrates, and raw biomass. The protein can be used for animal feed stocks, aquaculture feed stocks, and as a high quality protein source for human food and supplements. The carbohydrates can be fermented into bio ethanol. The remainder of the biomass can be utilized as fertilizers and as a solid fuel source.
PBR’s can be placed anywhere even underground if artificial lighting is used. The ideal location would be to place the PBR in direct proximity to an existing coal power plant or similar CO2 producing facility and pipe the CO2 directly into the PBR or storage connected to the PBR. This would provide mutual benefits and create a synergistic system where algae oil can be used to help power the plant providing the CO2. Some of the CO2 will be returned to the atmosphere when the oil is burned as a bio fuel but even that is in essence net carbon neutral since the CO2 was either absorbed by the algae in the form of CO2 already present in the atmosphere or absorbed from CO2 about to be released from a smoke stack into the atmosphere. Water can be used in the PBR that is otherwise unsuitable for normal farming with the consequence of lower yield expectations. Although, brackish, brine or wastewater is an excellent source for other essential nutrients like nitrogen, phosphorous, silicates, and sodium.
Bio algae production has a way to go before mass production expectations can be fulfilled. PBR efficiencies still require fine tuning. Government funding or subsidies would be a necessity especially for start up and small bio fuels companies. More research is required to isolate the most cost effective extraction processes. Despite these limitations, bio algae production from PBR’s represents one of the United States’ greatest opportunities for transition away from strictly fossil fuels, while providing a high protein food source for humans and as a feed stock for animal, poultry, and fish live stocks. It can also assist in the reduction of greenhouse gases by sequestering CO2. As production levels increase, PBR’s will be able to use their own oil output to run themselves removing the argument that it still requires fossil fuels to support bio fuel production.
There are future applications that may transcend even the current benefits. Possible applications include using bio diesel to fuel power plants and transitioning cars to electricity. Larger trucks can still remain powered on petrol diesel / bio diesel blends. CO2 emitted from using bio diesel to power the facilities could be reinserted back into the PBR creating a near closed loop CO2 sequestration system. Another application involves powering the steam reforming or electrolysis processes are to common methods used for hydrogen production. The CO2 emitted by both the steam reforming process and the bio diesel used to power that process could be fed back into the PBR system. This process has been traditionally powered by fossil fuels and criticized severely since the energy (usually fossil fuels) used to create the hydrogen is greater than the energy output of the hydrogen. Another even more potentially beneficial use would be to extract hydrogen direct from the algae during photosynthesis.
Please add to or make constructive corrections that will improve this blog.
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